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Immune responses to recombinant Brugia malayi pepsin inhibitor homolog (Bm-33) in patients with human lymphatic filariaisis

Identifieur interne : 005850 ( Main/Exploration ); précédent : 005849; suivant : 005851

Immune responses to recombinant Brugia malayi pepsin inhibitor homolog (Bm-33) in patients with human lymphatic filariaisis

Auteurs : N. S. A. Krushna ; C. Shiny ; G. Manokaran ; S. Elango ; S. Babu ; R. B. Narayanan

Source :

RBID : PMC:3515686

Descripteurs français

English descriptors

Abstract

Immune responses to recombinant Brugia malayi pepsin inhibitor homolog (rBm-33) were investigated in patients with human lymphatic filariasis (microfilaremics (MF) and chronic pathology (CP)) along with endemic normals (EN). Flow cytometric analysis (24 h) revealed CD4+ T cell activation in patients (MF and CP) compared to normals (EN), with increased expression of CD69 and diminished levels of CD62L and CD127. This was associated with an elevated expression of CD154 but not CD28 and CTLA4 in CP patients. However, Bm-33-induced cytokine expression profile (IL-1β, IL-12, IL-8, IFN-γ, IL-10 and TGF-β) did not exhibit any significant difference between normals and patients at the same time point. Although CD4+ T cell activation was observed initially in filarial patients (24 h), lymphoproliferation studies (96 h) suggested diminished proliferation compared to normals, indicating functional inactivation in the former upon prolonged antigen exposure. This indicates that rBm-33 induces an early T cell activation in MF and CP patients followed by a decreased lymphoproliferation that might contribute to immune suppression in these individuals.


Url:
DOI: 10.1007/s00436-010-2081-x
PubMed: 20927633
PubMed Central: 3515686


Affiliations:


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Le document en format XML

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<term>Elephantiasis, Filarial (drug therapy)</term>
<term>Elephantiasis, Filarial (immunology)</term>
<term>Gene Expression Regulation (drug effects)</term>
<term>Helminth Proteins (immunology)</term>
<term>Helminth Proteins (therapeutic use)</term>
<term>Humans</term>
<term>Immunity, Cellular (drug effects)</term>
<term>Immunity, Cellular (immunology)</term>
<term>Immunologic Factors (immunology)</term>
<term>Immunologic Factors (therapeutic use)</term>
<term>Leukocytes, Mononuclear (drug effects)</term>
<term>Leukocytes, Mononuclear (immunology)</term>
<term>Lymphocyte Activation (drug effects)</term>
<term>Lymphocyte Activation (immunology)</term>
<term>Recombinant Proteins (immunology)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>T-Lymphocytes (drug effects)</term>
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<term>ADN complémentaire (biosynthèse)</term>
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<term>ADN complémentaire (métabolisme)</term>
<term>Activation des lymphocytes ()</term>
<term>Activation des lymphocytes (immunologie)</term>
<term>Agranulocytes ()</term>
<term>Agranulocytes (immunologie)</term>
<term>Animaux</term>
<term>Anticorps antihelminthe (sang)</term>
<term>Antigènes d'helminthe (immunologie)</term>
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<term>Protéines d'helminthes (usage thérapeutique)</term>
<term>Protéines recombinantes (immunologie)</term>
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<term>Régulation de l'expression des gènes ()</term>
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<term>DNA, Complementary</term>
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<term>ADN complémentaire</term>
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<term>Elephantiasis, Filarial</term>
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<term>Cell Proliferation</term>
<term>Gene Expression Regulation</term>
<term>Immunity, Cellular</term>
<term>Leukocytes, Mononuclear</term>
<term>Lymphocyte Activation</term>
<term>T-Lymphocytes</term>
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<keywords scheme="MESH" qualifier="drug therapy" xml:lang="en">
<term>Elephantiasis, Filarial</term>
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<term>ADN complémentaire</term>
<term>Cytokines</term>
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<term>Activation des lymphocytes</term>
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<term>Antigènes d'helminthe</term>
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<term>Protéines d'helminthes</term>
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<term>Elephantiasis, Filarial</term>
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<front>
<div type="abstract" xml:lang="en">
<p id="P1">Immune responses to recombinant
<italic>Brugia malayi</italic>
pepsin inhibitor homolog (rBm-33) were investigated in patients with human lymphatic filariasis (microfilaremics (MF) and chronic pathology (CP)) along with endemic normals (EN). Flow cytometric analysis (24 h) revealed CD4
<sup>+</sup>
T cell activation in patients (MF and CP) compared to normals (EN), with increased expression of CD69 and diminished levels of CD62L and CD127. This was associated with an elevated expression of CD154 but not CD28 and CTLA4 in CP patients. However, Bm-33-induced cytokine expression profile (IL-1β, IL-12, IL-8, IFN-γ, IL-10 and TGF-β) did not exhibit any significant difference between normals and patients at the same time point. Although CD4
<sup>+</sup>
T cell activation was observed initially in filarial patients (24 h), lymphoproliferation studies (96 h) suggested diminished proliferation compared to normals, indicating functional inactivation in the former upon prolonged antigen exposure. This indicates that rBm-33 induces an early T cell activation in MF and CP patients followed by a decreased lymphoproliferation that might contribute to immune suppression in these individuals.</p>
</div>
</front>
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<name sortKey="Babu, S" sort="Babu, S" uniqKey="Babu S" first="S." last="Babu">S. Babu</name>
<name sortKey="Elango, S" sort="Elango, S" uniqKey="Elango S" first="S." last="Elango">S. Elango</name>
<name sortKey="Krushna, N S A" sort="Krushna, N S A" uniqKey="Krushna N" first="N. S. A." last="Krushna">N. S. A. Krushna</name>
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